MDA-kb2

The MDA-kb2 cell line is a human breast cancer cell line derived from an adult patient. These cells are estrogen receptor (ER) negative and androgen receptor (AR) positive, making them valuable for studies involving androgen signaling pathways and their implications in breast cancer. The MDA-kb2 cell line was derived from the breast cancer cell line, MDA-MB-453, by stable transfection with a mouse mammary tumor virus (MMTV) luciferase-neo reporter gene construct. This genetic modification allows for the use of MDA-kb2 cells in bioassays for androgenic and anti-androgenic activities, where they are often utilized in luciferase reporter assays due to their stable transfection with a luciferase reporter gene under the control of an androgen-responsive promoter. Due to their specific receptor profile, MDA-kb2 cells provide a crucial model for investigating the role of androgens in breast cancer progression and for testing the efficacy of potential therapeutic agents targeting AR pathways. These cells are cultured in Leibovitz's L-15 medium supplemented with 10% fetal bovine serum, under conditions that do not require CO2 supplementation, which is an atypical characteristic compared to many other cell lines. The unique properties of MDA-kb2 cells make them an indispensable tool in both basic research and pharmaceutical development, particularly in understanding hormone receptor interactions in breast cancer. Breast cancer cell lines, Breast, Mammary gland, Breast adenocarcinoma, Growth Conditions: Adherent, Biosafety level: BSL 1