MonoMac6

"The MonoMac6 cell line is a human monocytic cell line derived from a patient with monoblastic leukemia. It was developed to represent mature monocytes in vitro and serves as a robust model for studying monocyte biology and immune responses. Unlike earlier monocytic cell lines, such as U937 and THP-1, MonoMac6 inherently expresses markers and functions associated with mature monocytes without requiring differentiation induction. These include CD14 antigen, lysozyme production, and the capability for phagocytosis of antibody-coated erythrocytes. The cell line also demonstrates NaF-sensitive esterase activity, a hallmark of monocyte lineage, further validating its use as a model for mature monocyte behavior. Functionally, MonoMac6 cells are notable for their ability to produce cytokines such as TNF-α, IL-1β, and IL-6 in response to stimuli like lipopolysaccharides (LPS) and phytohemagglutinin A (PHA). This cytokine response closely mirrors that of primary human monocytes, though the cell line does not produce interferon-alpha (IFN-α), even when exposed to potent viral inducers. This feature makes MonoMac6 an essential tool for investigating specific monocyte-driven immune pathways and cytokine-mediated processes, including inflammation and sepsis. MonoMac6 cells exhibit extensive characterization at the morphological and cytogenetic levels. These cells display euchromatin-rich nuclei, a well-developed Golgi apparatus, and numerous lysosomes and endocytic vesicles, all consistent with the functional attributes of mature monocytes. The line's stable cytokine profiles and phenotypic markers make MonoMac6 an indispensable model for research into monocyte-related immunology, infections, and hematological malignancies." Leukemia cell lines, Peripheral blood, Adult acute monocytic leukemia, Growth Conditions: Suspension, singular cells and slightly adherent, small aggregates, Biosafety level: BSL 1