abberior LIVE 550- Halo

abberior LIVE 550 is a member of a new class of fluorescent dyes for live-cell imaging exclusively offered by abberior. This dye is highly photostable and bright orange fluorescent dye, which can be effectively excited with a 561 nm laser. For STED, a depletion wavelength between 650 - 775 nm is recommended. Due to the unique isomeric structure the favorited conformation is stabilized, leading to an enhanced cell permeability of the dye. abberior LIVE 550 can therefore be used in very low nanomolar concentration, reducing background and cytotoxicity effectively, allowing long-term STED and confocal imaging of living cells or tissue. Our abberior LIVE 550 is the ideal partner for abberior LIVE 610 to obtain optimal 2 color live-cell STED and confocal results. Discover our abberior LIVE 550 Halo, a fluorogenic label that covalently binds to the active site of your HaloTag fusion protein within living cells. This label rapidly binds with exceptional specificity under typical physiological conditions. Each purchase includes 1 vial of lyophilized abberior LIVE 590 Halo, ensuring top-quality performance for live-cell STED and confocal microscopy. For optimal use, we recommend a stock solution of 1 mM in DMF or DMSO. Adjust the working solution concentration between 0.1 µM to 0.5 µM based on the cell type, tissue, and expression rate of your HaloTag fusion protein.

DOSTUPNÉ FORMÁTY:

:

• Halo
• SNAP
• DNA
• tubulin
• actin
• click
• carboxylic acid