abberior LIVE 590- actin

abberior LIVE 590 is a member of a new class of fluorescent dyes for live-cell imaging exclusively offered by abberior. This dye is highly photostable and bright orange fluorescent dye, which can be effectively excited with excitation light between 550 - 610 nm. For STED, a depletion wavelength between 750 - 800 nm is recommended. Due to the unique isomeric structure the favorited conformation is stabilized, leading to an enhanced cell permeability of the dye. abberior LIVE 590 can therefore be used in very low nanomolar concentration, reducing background and cytotoxicity effectively, allowing long-term STED and confocal imaging of living cells or tissue. Cell permeable and photostable fluorescent probe used to label actin in fixed and living cells. This highly specific actin probe is based on Jasplakinolide. This product contains 1 vial of abberior LIVE 590 actin as lyophilized powder, a stock solution of 1 mM in DMF or DMSO is recommended. Depending on cell type or tissue a working solution of 1 nM to 100 nM is advised. Additionally, a vial containing 1 µmol verapamil is provided as an imaging reagent. 10 to 20 nmol verapamil in 1 ml staining solution increases the signal intensity significantly.

DOSTUPNÉ FORMÁTY:

:

• Halo
• HaloX
• SNAP
• DNA
• tubulin
• actin
• click
• carboxylic acid