NCI-H157 Cells

NCI-H157 is a human non-small cell lung carcinoma (NSCLC) cell line, primarily used in cancer research to study tumorigenesis, chemotherapy resistance, and the molecular pathways involved in lung cancer progression. NCI-H157 cells are particularly useful for investigating the role of hypoxia-inducible factor-1 alpha (HIF-1α) in NSCLC. Studies have shown that HIF-1α plays a crucial role in promoting angiogenesis, proliferation, and survival of cancer cells under hypoxic conditions. Downregulation of HIF-1α via siRNA in NCI-H157 cells significantly reduces cell proliferation, induces apoptosis, and impairs the invasive ability of the tumor cells. Moreover, combination treatments using HIF-1α siRNA and chemotherapy agents, such as cisplatin (DDP), enhance the cytotoxic effects on NCI-H157 cells. The reduction of HIF-1α expression has been shown to increase the activity of apoptotic proteins like caspases 3 and 9 while decreasing the levels of anti-apoptotic proteins such as Bcl-2. Additionally, HIF-1α knockdown inhibits key signaling pathways involved in tumor growth, including the PI3K/AKT and Raf/MEK/ERK pathways. These molecular alterations contribute to the suppression of tumor cell survival and invasiveness. The NCI-H157 cell line is also responsive to various natural compounds and plant extracts. For example, extracts from *Stellera chamaejasme* L. have been found to induce apoptosis in NCI-H157 cells through the Fas death receptor pathway, further emphasizing the cell line's utility in evaluating novel therapeutic agents for lung cancer. Lung cancer cell lines, Lung, Lung squamous cell carcinoma, Growth Conditions: Adherent, Biosafety level: BSL