U2OS-CRISPR-NUP96-Halo Cells

The U-2 OS-CRISPR-NUP96-Halo is a genetically engineered cell line derived from the human osteosarcoma U-2 OS cells. This cell line has been modified using CRISPR-Cas9 technology to incorporate a HaloTag at the NUP96 gene locus. NUP96, part of the nuclear pore complex, plays a critical role in nuclear transport and cellular regulation. The introduction of the HaloTag allows for the precise visualization and biochemical characterization of NUP96's dynamics and interactions within the cell. By facilitating the covalent attachment of fluorescent ligands or other probes, the HaloTag enables real-time imaging and provides a powerful tool for studying the nuclear transport mechanisms in living cells. This particular clone, number 252, has been selected for its stable expression of the HaloTagged NUP96, ensuring consistent performance in experimental setups. This feature makes it highly suitable for high-resolution imaging techniques and molecular interaction studies, thereby supporting advanced research in cellular biology, particularly in the context of nuclear function and genetic regulation.