HK-2xZFN-mEGFP-Nup107 Cells

The HK-2xZFN-mEGFP-Nup107 cell line is a genetically engineered variant of the Hela Kyoto cell line, derived from human cervical cancer cells. This cell line has been modified using Zinc Finger Nuclease (ZFN) technology to integrate monomeric Enhanced Green Fluorescent Protein (mEGFP) into the Nup107 gene, a crucial component of the nuclear pore complex (NPC). Nup107 plays a key role in nucleocytoplasmic transport, essential for cellular homeostasis and gene regulation. The mEGFP integration enables visualization and tracking of Nup107, facilitating studies on the NPC's dynamics and functions. This fluorescent tagging helps understand Nup107's spatial and temporal distribution and its interactions with other nucleoporins and transport factors. The HK-2xZFN-mEGFP-Nup107 cell line is invaluable for researching cellular transport mechanisms and disease pathophysiology. This cell line provides a robust model for studying the NPC's intricate workings and its health and disease implications, combining the genetic stability and human origin of Hela Kyoto cells with advanced genetic engineering.