U2OS-CRISPR-NUP96-mEGFP Cells

The U-2 OS-CRISPR-NUP96-mEGFP is a genetically modified cell line derived from the human osteosarcoma U-2 OS parent line. This cell line incorporates a targeted insertion of the monomeric Enhanced Green Fluorescent Protein (mEGFP) tag at the NUP96 gene locus, achieved through the CRISPR-Cas9 gene-editing technology. NUP96, part of the nuclear pore complex, is essential for nuclear transport, and its fusion with mEGFP allows for real-time visualization of nuclear pore dynamics under fluorescent microscopy, providing valuable insights into nuclear transport mechanisms and nucleocytoplasmic trafficking. This specific clone, numbered 195, has been selected for its stable expression of the NUP96-mEGFP fusion protein and maintains the typical characteristics of the U-2 OS lineage, including a robust cytoskeletal structure which is critical in studies related to cancer cell migration and metastasis. The application of CRISPR technology ensures precise gene editing, minimizing off-target effects which could compromise the integrity of the experimental outcomes. This makes U-2 OS-CRISPR-NUP96-mEGFP clone no.195 particularly useful for high-resolution imaging techniques and detailed cellular architecture studies, aiding in advanced research in cellular biology, cancer research, and nuclear transport phenomena.