GIMEN Cells

The GIMEN cell line is derived from the bone marrow metastasis of a young child diagnosed with stage IV neuroblastoma. These cells are classified as N-type, which typically indicates a neuroblastic phenotype characterized by high cell density, neuronal properties, and the capability for extensive neurite outgrowth in culture. The establishment of the GIMEN cell line provides a valuable model for studying the molecular and cellular mechanisms underlying aggressive forms of neuroblastoma, particularly those associated with metastatic dissemination. Functionally, GIMEN cells exhibit notable interactions with various cytokines and growth factors. Specifically, their growth is inhibited by interferon-gamma (IFN-gamma), a cytokine known for its antiproliferative effects on certain cancer cells. Furthermore, fibroblast growth factor-2 (FGF-2) demonstrates an antimitogenic effect on these cells, which can be reversed by the addition of IFN-gamma. This reversal suggests a complex interplay between these factors in modulating cell proliferation. Additionally, interleukin-1 beta (IL-1 beta) enhances the antimitogenic effects of FGF-2, indicating its potential role in the regulation of tumor growth dynamics in the neuroblastoma microenvironment. These interactions highlight the GIMEN cell line's utility in exploring the impact of cytokines and growth factors on neuroblastoma progression and response to therapy.