NCI-H1299 Cells

NCI-H1299, also known as H1299, is a cell line established from a lymph node metastasis of the lung from a 43-year-old white male patient with carcinoma. H1299 and H292 are non-small cell lung cancer (NSCLC) cell lines. Regarding their genetic profile, H1299 cells have a homozygous partial deletion of the p53 protein and lack expression of p53 protein. While KRAS mutations are commonly found in various types of cancer, including NSCLC, H1299 expresses KRAS WT. A549 is another NSCLC cell line that homozygously expresses endogenous KRAS G12S. Understanding the biology of KRAS and its downstream signalling pathways is crucial for developing effective cancer therapies. Therefore, this epithelial-like cell line is commonly used in cancer and immuno-oncology research. The morphology of H1299 cells is characterized by adherent flattened cells with a thickness of fewer than 5 microns. H1299 cells have an approximate doubling time of 22 - 30 hours. H1299 cells express keratin and vimentin but are negative for neurofilament triplet protein. They are also reported to be able to synthesize the peptide neuromedin B (NMB) at 0.1 pmol/mg protein but not the gastrin-releasing peptide (GRP). Compared to A549 cells with more epithelial characteristics, H1299 cells have more mesenchymal characteristics and less effective epithelial marker expression.