HK-CRISPR-mEGFP-Nup153 Cells

The HK-CRISPR-mEGFP-Nup153 cell line is a human cell model engineered to study nuclear pore complex (NPC) dynamics. Using CRISPR-Cas9, this cell line integrates a monomeric enhanced green fluorescent protein (mEGFP) tag into the Nup153 gene, a critical component of the NPC. This allows real-time visualization of NPC behavior in live cells. This cell line is useful for studying NPC assembly, disassembly, and their role in nucleocytoplasmic transport, mRNA export, and nuclear envelope integrity. The precise genome editing with CRISPR technology ensures reliable results, making this cell line valuable for understanding NPC functions in both normal and disease conditions.