HK-CRISPR-mEGFP-Nup214 Cells

The HK-CRISPR-mEGFP-Nup214 cell line is designed to study Nup214, a key nuclear pore complex (NPC) component. Using CRISPR-Cas9, this cell line adds a monomeric enhanced green fluorescent protein (mEGFP) tag to Nup214, allowing real-time visualization of its role in nucleocytoplasmic transport and cell cycle regulation. This cell line ensures minimal off-target effects and enables advanced imaging techniques to study NPC structure and function. It is valuable for investigating nucleocytoplasmic transport and related diseases, such as certain leukemias, and is useful for screening compounds that affect NPC function.