A9 Cells

A9 cells are a fibroblast-like cell line derived from mouse adipose tissue. They were established as a subclone of the L929 parent strain by W. R. Earle in 1940. The parent strain was obtained from normal subcutaneous areolar and adipose tissue of a male C3H/An mouse. A notable feature of these cells is that they express adenosine phosphoribosyl transferase (APRT) and hypoxanthine phosphoribosyl transferase (HPRT), denoted as APRT+ and HPRT+. These cells have been valuable in virus studies, particularly involving pseudorabies virus (PRV), vesicular stomatitis virus (VSV) of the Indiana strain, and herpes simplex virus (HSV). A9 cells' sensitivity and response to these viruses have made them useful for studying viral replication, pathogenesis, and potential antiviral treatments. In immunology, A9 cells are used in various research areas. They are a valuable model for studying immune responses, antibody production, monoclonal antibody generation, and hybridoma technology. Due to their rapid proliferation (doubling time of approximately 24 hours), A9 cells provide a sufficient cell supply for experiments and downstream applications. A9 cells have a fibroblast-like morphology and adhere to the culture substrate. Categorized as animal cells and belonging to the hybridoma cell type, A9 cells were formed by fusing B lymphocytes from Mus musculus (mouse) with myeloma cells from the same species. This unique combination allows A9 cells to exhibit properties of both B lymphocytes and myeloma cells. Overall, A9 cells are a well-established fibroblast-like cell line utilized for studying viral infections, especially PRV, VSV, and HSV, and in immunology.