J774A.1 Cells

The J774A.1 cell line was derived from the ascites tumour of a female BALB/c/NIH mouse during plasmacytoma-inducing treatment. The cells are known for their ability to perform antibody-dependent phagocytosis, making them a helpful tool for investigating immune responses to various antigens. The growth of J774A.1 cells is inhibited by various substances, including dextran sulfate, p-phenylenediamine (PPD), and lipopolysaccharide (LPS). J774A.1 cells synthesize large amounts of lysozyme and are known to synthesize interleukin-1 beta continuously.J774A.1 cells have a doubling time of 17 hours and can be cultured under the same conditions as RAW 264.7 macrophages. Additionally, the J774A.1 cell line is known to express specific genes, including interleukin-1 (IL-1) and lysozyme, as well as specific expression markers, such as complement (C3) and high-affinity Fc receptor, IgG (Fcgr1). The J774A.1 cell line has been used in various immunology and infectious disease studies. For example, it has been used to investigate the cytotoxicity of triazolo[1,5-a]pyridinium salts with leishmanicidal activity and the antitrypanosomatic activity of flavonoid glycosides isolated from Delphinium species. Overall, J774A.1 cells are a valuable tool in studying macrophage function, cytokine synthesis, and the immune response to various antigens and pathogens.