CCRF-CEM Cells

CCRF-CEM cells are a type of human T lymphoblasts commonly used in immuno-oncology and immunology research. These cells were isolated from the peripheral blood of a 4-year-old female Caucasian with acute lymphoblastic leukemia (ALL). CCRF-CEM grow in suspension and can reach high cell density when cultured in spinner flasks. Karyotype analysis of CCRF-CEM cells showed a modal number of 47 chromosomes, ranging from 41 to 95. They show no consistent loss or gain of specific chromosomes and no marker chromosomes. However, 28% of cells with 45 chromosomes showed C- and 53% of all cells had an extra D, and 35% had an additional F. CCRF-CEM cells are tumorigenic and can cause tumours in Syrian hamsters. These cells express CD3, CD5, CD7, and CD4 genes and antigens. Additionally, isoenzyme analysis showed ADA, 1; ES-D, 1; G6PD, B; GLO-I, 1; PEP-D, 1; PGD, C; PGM1, 1; PGM3, 0. These cells are reported to be free of virus particles as determined by electron microscopy. A study has shown that the combination of resveratrol and prednisolone induced apoptosis in CCRF-CEM cells in a time- and dose-dependent manner. The combination treatment showed synergistic effects on the overexpression of BAx and the downregulation of BCL2.