HK-ZFN-AURKB-mEGFP/ZFN-INCENP-mCherry Cells

The HK-ZFN-AURKB-mEGFP/ZFN-INCENP-mCherry cell line, derived from HeLa Kyoto cells, is a specialized model used in cellular biology research. It has been genetically engineered to express Aurora B kinase (AURKB) tagged with monomeric enhanced green fluorescent protein (mEGFP) and Inner Centromere Protein (INCENP) tagged with mCherry. These modifications enable researchers to track the dynamics and interactions of these proteins during cell division. Aurora B kinase is essential for chromosome segregation and cytokinesis, while INCENP is a critical component of the Chromosomal Passenger Complex (CPC), coordinating mitotic progression. This dual fluorescent tagging provides a powerful tool for live-cell imaging, allowing detailed study of protein distribution during the cell cycle. The HK-ZFN-AURKB-mEGFP/ZFN-INCENP-mCherry cell line is valuable for researching mitotic regulation, chromosomal stability, and the mitotic checkpoint. The precision of zinc finger nucleases (ZFNs) used for genetic modifications ensures the accuracy of this model, making it ideal for high-fidelity studies in cancer biology and therapeutic development.