EB3 Cells

The EB3 cell line is a mouse embryonic stem (ES) cell line that has been extensively studied for its response to cell?cell contact. One of the key features of the EB3 line is its expression of connexin30.3 (Cx30.3), a connexin isoform specifically expressed in the undifferentiated state of these cells. Cx30.3 expression is highly responsive to cell?cell contact, as demonstrated in experiments where hanging drop culture, which increases cell?cell interaction, led to a significant 1.73-fold increase in Cx30.3 expression compared to single cell cultures. This contact-dependent upregulation of Cx30.3 is mediated through the E-cadherin signaling pathway, and knockdown experiments with ?-catenin, a downstream factor of cadherin signaling, have confirmed its involvement. Inhibition of E-cadherin or β-catenin led to a reduction in Cx30.3 expression, indicating that cell?cell contact activation of Cx30.3 is at least partially regulated by this pathway. Interestingly, this cell contact response is specific to connexin30.3, as the expression of connexin43 (Cx43), another connexin isoform, did not exhibit the same level of sensitivity to cell contact in these experiments. The EB3 cell line is a useful model for studying the mechanisms of cell?cell communication and the role of gap junctions in early embryonic development. The unique expression pattern of Cx30.3 in response to contact suggests that it may play a role as a signaling mediator during tissue morphogenesis and differentiation in the undifferentiated stem cell state. Burkitt Lymphoma cell lines, Bone, Burkitt lymphoma, Growth Conditions: Suspension, Biosafety level: BSL 2