iPSC-hDPSC

The iPSC-hDPSC cell line represents a cutting-edge model derived from human dental pulp stem cells (hDPSCs). These cells originate from dental pulp tissue, where they undergo enzymatic digestion to isolate the adherent fraction. Upon reaching 70-80% confluence, the cells are subcultured and cryopreserved at Passage 1. This foundational step is crucial in maintaining the cellular integrity and ensuring the viability of the stem cells for future applications. The reprogramming of hDPSCs into induced pluripotent stem cells (iPSCs) is a significant advancement, achieved through an episomal reprogramming technique. This method employs vectors that incorporate the Yamanaka factors-Oct-4, Sox-2, and Klf-4-alongside p53 Anti-sense, EBNA-1, and a Red Fluorescent Protein marker. The episomal approach is particularly advantageous as it avoids the integration of reprogramming factors into the genome, thus preserving the genetic stability of the iPSCs. The resultant iPSC-hDPSC line exhibits pluripotent characteristics, making it a valuable tool for research in regenerative medicine, disease modeling, and drug discovery.