HK-CRISPR-CAP-H-mEGFP Cells

The HK-CRISPR-CAP-H-mEGFP cell line is a human-derived model engineered for advanced gene editing and fluorescence applications. This cell line is based on a parental human cell line and has been modified using CRISPR-Cas9 technology to express a CAP-H (Chromosome-Associated Protein H) gene tagged with monomeric Enhanced Green Fluorescent Protein (mEGFP). This modification allows for precise visualization and tracking of CAP-H, a component of the condensin complex crucial for chromosome condensation and stabilization during cell division. The mEGFP tag provides a strong and stable fluorescence signal, making this cell line ideal for live-cell imaging and fluorescence-based assays. The HK-CRISPR-CAP-H-mEGFP cell line is particularly valuable for studies in cell cycle regulation, mitosis, and chromosomal dynamics. Researchers can utilize this model to investigate the roles of condensin complexes in maintaining chromosomal integrity, especially during critical phases such as metaphase and anaphase. The stable integration of the mEGFP tag ensures consistent expression and reliable experimental outcomes, enhancing reproducibility across different studies.