DH82 Cells

DH-82 cells, derived from the malignant histiocytosis of a ten-year-old male Golden Retriever, are a cornerstone in the study of canine immunology and related diseases. These cells exhibit a macrophage-like morphology, mirroring the key functions of human macrophages, thereby providing a relevant model for investigating various aspects of canine health, particularly immune system-related conditions. A defining characteristic of DH-82 cells is their capability to phagocytize latex particles, an essential function of macrophages responsible for the elimination of foreign substances in the body. This property positions DH-82 cells as a robust tool for delving into the immune responses of dogs, especially in the face of infections and inflammatory diseases. The expression of Fc gamma receptors in DH-82 cells is a notable trait. These receptors are integral to immune responses, as they bind to antibodies and facilitate the phagocytosis of antibody-coated pathogens or particles. This makes DH-82 cells particularly valuable in studies focusing on immune responses and antibody-dependent cellular cytotoxicity (ADCC). In contrast, DH-82 cells do not express Fc mu and C3b receptors. The absence of Fc mu receptors, typically found on B cells and involved in antigen presentation, and C3b receptors, which bind to complement proteins in immune responses, provides a controlled setting for examining specific immune mechanisms that might be influenced by these receptors. Additionally, DH-82 cells are non-producers of IL-1, a pivotal cytokine in inflammatory responses. This feature offers a unique perspective for investigating the role of IL-1 in various biological processes and understanding IL-1-mediated diseases. In the realm of infectious diseases, DH-82 cells have proven particularly useful in studying canine monocytic ehrlichiosis (CME), a tick-borne illness caused by Ehrlichia canis. The cells provide a conducive environment for the bacterium's growth, aiding in the exploration of the disease's development and potential treatments. The doubling time of DH-82 cells, approximately 26 hours, is also a critical aspect in their use, influencing experimental design and the interpretation of results.