CFPAC-1 Cells

CFPAC-1 cells, derived from a 26-year-old male with cystic fibrosis and liver metastasis of ductal adenocarcinoma, are a hyperdiploid cell line with notable features for biological research. Their adherence growth property and tumorigenic capability in nude mice make them a practical model for in vitro cancer studies. The cell line's karyotype includes a modal number of 73 chromosomes with several translocations, and importantly, two to three copies of chromosome 7, where the cystic fibrosis gene is located. These cells express cancer-related antigens and genes like CA19-9, carcinoembryonic antigen (CEA), pancreatic oncofetal antigen (POA), adenocarcinoma associated antigen (ACAA), and epithelial keratins, offering insights into cancer biology. In terms of cystic fibrosis pathology, CFPAC-1 cells demonstrate unique ion transport activities. They do not respond to cAMP agonists, adenyl cyclase stimulators, or phosphodiesterase inhibitors for chloride ion flux but show increased chloride efflux in response to calcium ionophores. CFPAC-1 cells carry the common cystic fibrosis mutation - deletion of three nucleotides leading to phenylalanine absence at position 508 in the CFTR gene. Morphologically, they exhibit epithelial features with apical microvilli, tight junctions, and gap junctions, relevant for studying epithelial tissue interactions in both cancer and cystic fibrosis.