RM-1 Cells

The RM-1 cell line is a fibroblast-like model derived from the embryonic prostate tissue of C57BL/6 mouse embryos. These cells were isolated from 17-day-old urogenital sinus cells and infected with the Zipras/myc9 retrovirus, containing the oncogenes v-Ha-Ras and v-Myc. RM-1 cells exhibit high tumorigenic potential, forming poorly differentiated mouse prostate carcinomas with high frequency. RM-1 cells are positive for cytokeratin 18 mRNA and immunoreactive to cytokeratin-specific antiserum, indicating epithelial origin. They demonstrate significant mitogenic response to testosterone, with approximately a two-fold increase in cell number under serum-free conditions. This cell line maintains stable androgen receptor numbers and binding kinetics across multiple passages, making it a valuable model for studying androgen sensitivity and prostate cancer progression.