NCI-H295R Cells

H295R was adapted from the NCI-H295 pluripotent adrenocortical carcinoma cell line established by A.F. Gazdar and associates (1990) from a carcinoma of the adrenal cortex. The original cells were adapted to a culture medium which decreased the population doubling time from 5 days to 2 days. The adapted cells were selected to grow in a monolayer, in contrast to the original cells which grew in suspension. This cell line retains the ability to produce adrenal androgens. It is responsive to angiotensin II and potassium ions. Adrenal gland cancer cell lines, Adrenal gland, Carcinoma, Growth Conditions: Monolayer, adherent, Biosafety level: BSL 1