Practical Workshop on SMLM: Advanced Imaging with Abbelight SAFeMN360
This two-day hands-on workshop will provide a practical introduction to Single-Molecule Localization Microscopy (SMLM) and its applications in super-resolution imaging. In addition to introductory lectures and practical experiences from experienced SMLM users, participants will work directly with the Abbelight SAFeMN360 system.

The practical sessions will cover the complete SMLM workflow, including sample preparation, image acquisition, and image analysis, allowing participants to gain hands-on experience with the key steps of SMLM experiments. The workshop will also address practical aspects of sample quality control and experimental setup.
Dates: November 11 and 12, 2026
Venue: Military Faculty of Medicine of the University of Defence, University Hospital Hradec Králové campus, Building No. 55
Registration fee: 1,000 CZK, including VAT. Fifteen participants will be selected and divided into three groups.
Included in the course fee: refreshments during coffee breaks and lunch
Not included: transportation and lodging
| Group 1 | Group 2 | Group 3 | ||
| Wed 11 Nov | 9:00 - 10:00 | Yashar Rouzbahani (Abbelight) General SMLM Introduction |
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| 10:00 - 10:30 | Christian Franke (Uni Jena) - online SMLM in the broader context of super - resolution microscopy. |
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| 10:30 - 11:00 | Coffee Break | |||
| 11:00 - 13:00 | Sample Prep | Image Acq. | Image Analysis | |
| 13:00 - 14:30 | Lunch | |||
| 14:30 - 15:00 | Jakub Pospíšil (TU Dresden) From Sample to Reliable SMLM: Practical Tips and Quality Control |
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| 15:00-17:00 | Image Acq. | Image Analysis | Sample Prep | |
| Group 1 | Group 2 | Group 3 | ||
| Thu 12 Nov | 9:00 - 9:30 | Delgir Zakinova (Uni Jena) To be confirmed |
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| 9:30 - 11:30 | Image Analysis | Sample Prep | Image Acq. | |
| 11:30-12:30 | Coffee Break | |||
| 12:30 - 13:00 | Peter Hoboth (IMG Prague) Nuclear Nanoscale - practical experiences with SMLM |
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| 13:00-14:00 | Wrap-up session | |||
| 14:00 | Lunch | |||

