abberior STAR ORANGE, FluoTag®-X4 anti-GFP, 200 µl (2.5 µM)

abberior STAR ORANGE is a novel fluorescent dye developed for STED and confocal microscopy in the orange spectral region. Introducing negatively charged groups into its molecular structure making the dye excellently water soluble and enables background-free imaging. Thus, abberior STAR ORANGE is highly photostable and a bright orange fluorescent dye, which can be effectively excited with excitation light between 550 - 610 nm. For STED microscopy, this dye can be most efficiently used with a STED laser wavelength between 750 - 800 nm. Our abberior STAR ORANGE can substitute dyes like ATTO® 594 or Alexa Fluor® 594. Together with our abberior STAR RED you can obtain stunning 2 color STED results. In combination with our abberior STAR 580 you get the ideal pair for FLIM experiments. Best results are obtained with freshly prepared samples. Single-domain antibodies (sdABs), also known as nanobodies, represent an innovative and promising antibody format. At just 15 kDa, sdABs are 10 times smaller than conventional whole IgG antibodies, making them ideal for high-resolution imaging applications that require small labels, excellent tissue penetration, and exceptional specificity. The combination of abberior STAR dyes—known for their superior brightness and photostability—and FluoTag®-X4 anti-GFP from NanoTag provides an exceptional solution for high-quality, high-resolution imaging. FluoTag®-X4 anti-GFP, developed by NanoTag, is a unique blend of two sdABs that bind with high affinity and specificity to two distinct epitopes on GFP and its most common derivatives. This enables direct and highly specific fluorescent detection of GFP-tagged targets, eliminating the need for secondary antibodies. This product includes one abberior STAR ORANGE FluoTag®-X4 anti-GFP vial as a lyophilized reagent. Before staining, dissolve the reagent in 200 µl of sterile 50% glycerol (v/v) in deionized water. Please refer to our protocol for detailed instructions on the staining procedure, handling, and storage.

DOSTUPNÉ FORMÁTY:

• donkey anti-sheep
• donkey anti-goat IgG
• donkey anti-human IgG
• donkey anti-rat IgG
• donkey anti-guinea pig IgG
• donkey anti-rabbit IgG
• donkey anti-mouse IgG
• goat anti-rat IgG
• goat anti-guinea pig IgG
• goat anti-chicken IgY
• goat anti-rabbit IgG
• goat anti-mouse IgG
• nanobody FluoTag®-X2 anti-Rabbit IgG
• nanobody FluoTag®-X2 anti-Mouse IgG1
• FluoTag®-X4 anti-GFP
• membrane
• Halo
• streptavidin
• phalloidin
• Protein Labeling Kit
• maleimide
• carboxylic acid