abberior STAR RED, nanobody FluoTag®-X2 anti-Mouse IgG1, 500 µL (5 µM)

abberior STAR RED is a well-known red dye, named also KK114 in literature. It was developed for STED and confocal imaging in the red spectral region. It shows an exceptional brightness and low background, delivering stunning STED images. abberior STAR RED can be excited with lasers at wavelength between 630 - 650 nm. For STED, a depletion wavelength around 750 - 800 nm is recommended.abberior STAR RED can substitute dyes like ATTO® 647N, Alexa Fluor® 647, or Cy5®. Our abberior STAR RED is the ideal partner for abberior STAR ORANGE or abberior STAR 580 to obtain optimal 2 color STED results. Best results are obtained with freshly prepared samples. Secondary single-domain antibodies (sdABs), also known as nanobodies, represent an innovative and promising antibody format. At just 15 kDa, sdABs are 10 times smaller than conventional whole IgG antibodies, making them ideal for high-resolution imaging applications that require small labels, excellent tissue penetration, and exceptional specificity. The combination of abberior STAR dyes – known for their superior brightness and photostability – with secondary sdABs (FluoTag®-X2) from NanoTag Biotechnologies provides an exceptional solution for high-quality, high-resolution imaging. FluoTag®-X2 sdABs, developed by NanoTag Biotechnologies, bind with high affinity and specificity to a distinct epitope on mouse IgG1. Due to the inherent symmetry of the target antibody, two secondary nanobodies will be able to bind each primary antibody. The secondary FluoTag®-X2 reagents will therefore target 4 fluorophores to each primary antibody. This product includes one abberior STAR RED FluoTag®-X2 anti-Mouse IgG1 vial as a lyophilized reagent. Before staining, dissolve the reagent in 500 µl of sterile 50% glycerol (v/v) in deionized water to obtain a concentration of 2.5 µM of the nanobody (5 µM dye). Please refer to our protocol for detailed instructions on the handling, storage, and staining procedure.

DOSTUPNÉ FORMÁTY:

• donkey anti-sheep IgG
• donkey anti-goat IgG
• donkey anti-human IgG
• donkey anti-rat IgG
• donkey anti-guinea pig IgG
• donkey anti-rabbit IgG
• donkey anti-mouse IgG
• nanobody anti-rabbit IgG
• nanobody anti-mouse IgG
• goat anti-rat IgG
• goat anti-guinea pig IgG
• goat anti-chicken IgY
• goat anti-rabbit IgG
• goat anti-mouse IgG
• nanobody FluoTag®-X2 anti-Rabbit IgG
• nanobody FluoTag®-X2 anti-Mouse IgG1
• FluoTag®-X4 anti-GFP
• membrane
• DPPE
• DOPE
• Halo
• streptavidin
• phalloidin
• Protein Labeling Kit
• azide
• maleimide
• NHS
• carbonate
• carboxylic acid