abberior STAR 635P, Protein Labeling Kit, 1 Kit

abberior STAR 635P was the first member of an entirely new class of fluorescent dyes introduced exclusively by abberior: the class of phosphorylated fluorescent dye. This dye excels in its photophysical parameters, it is very bright, very photostable and its high water solubility enables basically background-free imaging. abberior STAR 635P can be excited with lasers at wavelength between 630 - 650 nm. For STED, a depletion wavelength around 750 - 800 nm is recommended. abberior STAR 635P is the ideal partner for abberior STAR 580 or abberior STAR ORANGE to obtain optimal 2 color STED results. Our abberior STAR 635P can substitute ATTO® 647N, Alexa Fluor® 647, or Cy5®. Best results are obtained with freshly prepared samples. Introducing our advanced protein labeling kit, ideal for seamlessly labeling various proteins including polyclonal and monoclonal IgG antibodies, Fab fragments, nanobodies, and more, with high-quality abberior dyes. Optimized for proteins over 4 kDa, this kit contains all the necessary components for a quick and straightforward labeling process. The included protocol ensures protein conjugates with exceptional brightness and photostability. The procedure has been thoroughly tested with our abberior dyes, which react with free amines on the protein to form stable amide bonds under mild conditions, providing consistent results. Note that this kit is not suitable for IgM proteins. While proteins smaller than 4 kDa can be labeled, purification using the included ultrafiltration system will need to be carried out separately.