abberior CAGE 552 NHS ester,1 mg

abberior CAGE 552 is a caged dye which is initially nonfluorescent and colorless substance which, upon photolysis at 360 - 440 nm, readily transforms into a red and highly fluorescent dye. It absorbs around 552 nm and emits around 575 nm. abberior CAGE 552 is designed for single-molecule photoswitching microscopy modes such as PALM, STORM and GSDIM and, in its uncaged form, also performs well in STED microscopy. After uncaging it can be effectively used for STED with a depletion wavelength between 650 - 775 nm. High brightness and a very good photostability are key features of the uncaged form of this dye. It is the smallest and lightest caged rhodamine dye available. Further, after photoactivation, abberior CAGE 552 can be tracked to analyze molecular dynamics such as diffusion, flow directions and velocities in tracking experiments (in which the fluorescence signal is observed over time after the initial release of the fluorophore). The NHS ester is a widespread tool for conjugating a fluorescent dye to a protein or antibody. NHS ester can be used to label primary amines of proteins or amine-modified molecules. The resulting abberior conjugate will show a bright fluorescence and photostability. abberior offers protocols for labeling antibodies via amine-reactive groups.