abberior CAGE 635, NHS ester, 250 µg

abberior CAGE 635 is a caged dye which is initially colorless and nonfluorescent. When illuminated with UV light it undergoes a rapid uncaging reaction and releases a highly fluorescent red dye. It can most effectively be excited from 620 to 645 nm. abberior CAGE 635 is designed for single-molecule photoswitching microscopy modes such as PALM, STORM and GSDIM and, in its uncaged form, also performs well in STED microscopy. Further, after photoactivation, abberior CAGE 635 can be tracked in confocal and epifluorescence microscopy to analyses molecular dynamics such as diffusion, flow directions and velocities, e.g. are tracking experiments in which the fluorescence signal is observed over time after the initial release of the fluorophore. The NHS ester is a widespread tool for conjugating a fluorescent dye to a protein or antibody. NHS ester can be used to label primary amines of proteins or amine-modified molecules. The resulting abberior conjugate will show a bright fluorescence and photostability. abberior offers protocols for labeling antibodies via amine-reactive groups.