abberior FLUX 640, nanobody anti-rabbit IgG, 50 µg

abberior FLUX 640 is a dye developed for MINFLUX imaging in the red spectral region. It combines exceptional photostability, high brightness, and low background labeling with photoswitching kinetics optimized for MINFLUX imaging. abberior FLUX 640 can be applied with excitation laser wavelengths between 630 - 650 nm together with a detection window between 650 - 750 nm. For MINFLUX imaging abberior FLUX 640 is used with MINFLUX imaging buffers such as GLOX buffer. Our abberior FLUX 640 can substitute dyes like Alexa Fluor® 647, or Cy5®. The combination of abberior FLUX 640 and abberior FLUX 680 is recommended for 2 color MINFLUX imaging. Traditional primary and secondary antibody techniques can result in a ~20 nm distance from your epitope of interest to the fluorophore, causing linkage error. For techniques like MINFLUX, which can localize fluorophores with <3 nm precision, minimizing this linkage error is crucial for accurate analysis. Our innovative solution combines abberior FLUX dyes with Jackson ImmunoResearch's polyclonal secondary nanobodies (AffiniPure-VHH Secondaries). Optimized for single molecule localization techniques such as MINFLUX and STORM microscopy, abberior FLUX dyes offer unparalleled precision. The nanobodies are cross-adsorbed for exceptional specificity, reducing cross-reactivity and making them ideal for multiple labeling experiments. This product contains a vial of abberior FLUX 640 nanobody anti-rabbit IgG with a 1 mg/ml concentration. Detailed staining procedures, handling, and storage instructions are provided in our protocol.

DOSTUPNÉ FORMÁTY:

• nanobody anti-rabbit IgG
• nanobody anti-mouse IgG
• goat anti-rabbit IgG
• goat anti-mouse IgG
• SNAP
• streptavidin
• Protein Labeling Kit
• maleimide
• carboxylic acid