AE-1 Cells

Derived from a fusion of Mus musculus B lymphocytes and myeloma cells, AE-1 hybridoma cells possess lymphoblast morphology and grow in suspension culture. The derivation of AE-1 cells involves immunizing animals with purified human erythrocyte acetylcholinesterase, followed by fusion with Sp2/0-Ag14 myeloma cells. AE-1 cells are specifically designed to target the antigenic determinant of human acetylcholinesterase. They express immunoglobulin and a monoclonal antibody against this enzyme, providing researchers with a powerful tool to investigate the immunological aspects related to acetylcholinesterase in humans. The isotype of AE-1 cells is IgG1, enhancing their binding affinity and functionality. These cells offer remarkable antibody specificity, reacting with human and monkey acetylcholinesterase but binding to a different epitope compared to AE-2 cells. This specificity enables precise targeting and evaluation of acetylcholinesterase-related processes in different species. In the field of immunology, AE-1 cells find various applications. Researchers can utilize them to study immune responses, antibody production, and antigen-antibody interactions. These cells play a crucial role in advancing areas such as vaccine development, autoimmune disease research, and immunotherapy. Mouse cell lines, Hybridoma, Growth Conditions: Suspension, Biosafety level: BSL 1